Microbial Genetics. Front Cover. Stanley R. Maloy, John E. Cronan, David Freifelder. Jones and Bartlett Publishers, - Microbial genetics - pages. Microbial Genetics, Part 8. Front Cover. David Freifelder Microbial Genetics · Stanley R. Maloy,John E. Cronan,David Freifelder No preview available - Microbial Genetics (Jones and Bartlett Series in Biology): Medicine & Health Science Books @ ronaldweinland.info by Stanley Maloy (Author).
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Stanley R Maloy; John E Cronan; David Freifelder. Stanley R. Maloy, John E. Cronan, Jr., David Freifelder. Add tags for "Microbial genetics". Jones and Bartlett Publishers, - Science - pages. The revision of this classic textbook by David Freifelder has been rewritten and updated to include the numerous and recent advances in microbial genetics. The basic format, organization and style of the first edition has. No eBook available Microbial genetics. Front Cover Microbial Genetics · Stanley R. Maloy,John E. Cronan,David Freifelder QR code for Microbial genetics.
There are two types of transduction: generalized transduction and specialized transduction. This process is experimental and the keywords may be updated as the learning algorithm improves. This is a preview of subscription content, log in to check access. Preview Unable to display preview. Download preview PDF. References 1.
Herpesviruses J. Clements and S.
Respiratory Tract Viruses C. The Interferons S. Westby and A. Buchbinder and N. Diagnostic Virology T. Antiviral Chemotherapy M.
Principles of Parasitology and Parasitic Disorders B. The Molecular Epidemiology of Parasites G.
Toxin-Induced Diseases J. The Pathogenesis of Sepsis D. Simon, L. Goodman, and R. Bacterial Meningitis C.
Sable and W. Armstrong and P. BioTechniques 2: — Google Scholar 7. Google Scholar 9.
Microbiol Rev — Google Scholar Poteete AR Bacteriophage P In: R Calender ed : The bacteriophages. Schmieger H Phage Pmutants with increased or decreased transduction abilities. In: R Calendar ed : The bacteriophages. Genetics 17—27 Google Scholar Genetics — Google Scholar J Bacteriol — Google Scholar J Virol 46—52 Google Scholar Welker NE Transduction in Bacillus stearothermophilus.
Thorne CB Transduction in Bacillus thuringiensis. In different studies, applications such as the construction of mutants, the isolation of insertion sequence IS elements, and the curing of plasmids have been described. The most-used counterselectable markers are the genes that confer sucrose, streptomycin, or fusaric acid sensitivity. They have been used to construct mutants or vaccine strains in Mycobacterium tuberculosis, Helicobacter pylori, Bordetella pertussis, and many other bacteria.
Here we provide a short review of the situations in which the use of a counterselectable marker has proven to be particularly advantageous.
The first counterselectable marker described was tetAR, a gene coding for tetracycline resistance or sensitivity. Expression of tetAR results in alteration of the host cell membrane which interferes with tetracycline permeation and thereby renders the cell resistant to tetracycline. These alterations render the bacteria hypersensitive to lipophilic chelating agents such as fusaric or quinalic acids 5 , It is therefore possible to select clones which have lost the tetAR gene by virtue of their resistance to fusaric acid.
This system is, to the best of our knowledge, effective only in Escherichia coli, in which the threshold of fusaric acid sensitivity is strongly dependent on the host strain.
As a consequence, this marker was mostly used for abrogating tetracycline resistance in E. One problem encountered with this system is the generation of out-of-frame deletions with polar effects on downstream genes, which obscure the interpretation of the phenotype. The streptomycin sensitivity system.
The streptomycin sensitivity system takes advantage of the fact that the S12 ribosomal protein is the target of streptomycin, a widely used antibiotic. Mutations in the rpsL gene which encodes this protein are responsible for resistance to high concentrations of streptomycin.
However, resistance is recessive in a merodiploid strain When both wild-type and mutant alleles of rpsL are expressed in the same strain, the strain is sensitive to streptomycin, possibly because of a general inhibition of translation by the wild-type ribosome.
Consequently, it is possible to select mutants that have lost the wild-type allele encoding streptomycin sensitivity by plating the culture on streptomycin Positive selection based on streptomycin sensitivity functions in different bacteria, such as E.