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High voltage Needle diameter are derived successfully from power supply numerous polymers . Because there are a Tablo 1. Table 1. XPS widely in the control of CO emission in cars. In this study, the electrospinning test mechanism installed at Afyon Kocatepe University Materials Science and Enginee- ring Department was used Figure 2. The electrospinning mechanism in the figure comprises 3 main components. High voltage power supply Feeding unet Collector plate As high voltage power supply, Gamma ES30 brand DC po- wer supply with no step voltage adjustment capability was used.
High voltage power supply Feeding unet Collector plate As high voltage power supply, Gamma ES30 brand DC po- wer supply with no step voltage adjustment capability was used. As feeding unit, Top Syringe Pump Top model syringe infusion pump was used. The flow rate provided by the device may be adjusted at increments of 0.
The solution is placed into a 10 ml 22 gauge stainless steel tip syringe and put into the feeding unit. Collector plates are placed under the feeding unit to form fibers.
The collector plate is chosen from amongst high conductivity metals. The aluminum coil placed onto the Figure 2.
Electrospinning test mechanism copper plate is connected to the power supply. Evaluation of Test Results 3. XRD Analysis 2. XRD Analiz It was observed that bead formation increased in the fibers as the rate of solution feeding was increased.
The lowest feeding rate has allowed nano- fibers of smallest diameters nm to be obtained. As the applied voltage parameters was changed, a uni- form change was observed in the diameters of fibers.
While as the applied voltage increased, the fiber diameter decre- ased down to a specific point, after that point, the increase in the voltage value causes more sol feeding. Louis, MO to a concentration of 7.
Mice were sacrificed by decapitation, and the brain removed and quickly immersed in ice-cold, carbogen bubbled artificial cerebrospinal fluid ACSF prior to vibratome slicing Vibratome Series , Technical Products International, St. Louis, MO. The cerebellum and inferior colliculus were removed with dissection scissors to create a flat tissue surface to allow vibratome stage mounting prior to slicing.
Once the whole mouse brain was on the stage, ice-cold carbogen bubbled ACSF was quickly transferred into the vibratome stage to immerse the whole brain. Culture inserts were prepared and placed in a 6-well plate containing pre-warmed culture medium 1. The slices were then cultured in a tissue culture incubator with medium replacement every two days.
Here we used mouse brain slices to demonstrate our microfluidic platform because organotypic slice cultures are well-established in neuroscience research 21 and have been used with great success and consistency by many groups 20 , 22 — We were able to confirm high viability of brain slices in our device after 2 and 7 days in culture using vital staining dyes prior to starting dose-dependent toxicity studies Supplementary Fig.
The cells were characterized for invasive and migratory behaviors both in vitro and in vivo and then confirmed for positive response to Temozolomide TMZ GBM8 cells were labeled by lentiviral GFP expression to allow for the identification of the cells prior to xenograft generation.
Xenograft slices were transferred to PTFE membrane well inserts for culture. The resulting GBM xenograft slices contained glioma cells spreading out in the mouse brain from the original injection site with a characteristic architecture Supplementary Fig. Device operation for drug screening The assembled microfluidic device was treated with oxygen plasma using the same conditions for bonding, sterilization and hydrophilization prior to use.
Immediately after the plasma treatment, the device was transferred to a cell culture hood and microchannels were filled by pipetting culture medium into well reservoirs, covering the open channels with a PTFE membrane and then applying suction to the outlet.
Once microchannels were filled, the device was left in a cell culture incubator for 1 hr to allow culture medium to temperature and pH equilibrate prior to use. Tissue slices were transferred from the porous membrane well insert by first cutting PTFE membrane and placing it onto the open microchannels of the device.
The full culture area of the device 80 parallel open channels was imaged to register the position of tissue slices relative to delivery channels. We found that mouse brain slices should be cultured for at least 3 days to reach peak viability and recover from cell damage caused by tissue preparation.
Tissue slices were then permeabilized with 0. An automated x-y stage and both 4X and 10X objectives were used to acquire fluorescent images. Individual images were focused manually during stitching.
Drug-treated and control regions were located visually using the binoculars of the microscope.
The thickness of optical sections was 2. Recently, a random Transparent and conducting materials are widely used in network of silver nanowires Ag NWs has been demonstrated optoelectronic applications such as liquid crystal displays to be a potentially good transparent conductor. Following this purpose. Despite its superior properties such as high fabrication.
Ag NWs could be coated onto different substrates optical transparency and low sheet resistance, it has some via various deposition processes [12—17]. Ag NW networks crucial and well-known drawbacks such as lack of flexibility show comparable transparency and sheet resistance values and a high price. Hence, a new transparent conductor to ITO; however, for ultimate replacement of ITO there are is desirable and various approaches have been reported a few more challenges with Ag NW networks yet to be including single-walled carbon nanotube networks [4, 5], resolved.
First, Ag NW networks do not form a continuous graphene [6, 7], metal grids , conducting polymers  film so that roughness values comparable to the diameter and metal thin films .
However, most of these still fail 60— nm of the NWs typically arise.
The roughness of 2. Fabrication of the PLEDs the networks can also cause a hazy appearance that is not desirable for some applications such as touch screens.
Poly 2-methoxy 2-ethylhexyloxy -1,4-phenylenevinylene Secondly, most conventional Ag NW synthesis routes involve MEH-PPV was purchased from Sigma Aldrich, chloroform polyvinylpyrrolidone PVP on the lateral surfaces of Ag NWs and poly 3,4 ethylenedioxythiophene —poly styrenesulfanate which increases the sheet resistance of the networks.
All chemicals methods to address these issues have been reported but a were used without further purification. For the hole transport cohesive approach that addresses both has yet to be elucidated.
Methods a nm pore size filter and spin coated on top of the Ag NW networks at different spin coating speeds. Immediately 2. In brief, in a standard synthesis experiment, emitting layer. In the solvent was evaporated. Device fabrication was finalized by meantime, a 0.
The solution was stirred at a rate of Technologies, Part No. Following the injection process, the solution was annealed for another 30 min.
Then, for purification, the Ag NW solution was diluted with acetone at a volume ratio of 3.
Results and discussion and centrifuged twice at 10 rpm for 15 min. Another centrifuge process was conducted with the same parameters Ag NWs were synthesized through a polyol process. These ethanolic solutions of Ag NWs were deposited onto glass substrates 2. Characterization using a simple air brush setup. A photograph of the Ag analysis of Ag NW networks on silicon substrates.
The NW networks deposited onto a glass substrate is shown tapping mode was used for the analysis.